Questions 1) Do th e direct and viable counts of the suspension agree? If not, w

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Questions 1) Do th e direct and viable counts of the suspension agree? If not, why might this be? ou dilute an original sample 1:30. You then count the number of yeast in the 1:30 dilution. You cou nt an average of 7 cells in the 1/25 mm2 sized boxes. What is the concentration, in cells/ml, of the original sample? 3) You count the number of bacteria in 5 of the 1/400 mm2 small boxes of the central grid on the hemocytometer. Your results are: 12 cells in box # 1,1 7 cells in box #2, I 7 cells in box #3, 14 cells in box #4, and 16 cells in box #5. You are counting a 1-10 dilution of the original sample. What is the concentration of the original culture (in cells/ml)? 4) You count 74 cfu on a spread plate. The plate was prepared by spreading 0.2 ml of a 1:10,000 dilution of the original sample. What is was the concentration of the original culture (in cells/ml)? You have a culture of yeast that is at a concentration of 4.76 x 10s cells/ml. You dilute the sample 1:100, and then 1:100 again, and finally you dilute the sample an additional 1:3. You add 0.1 ml of the final dilution to a spread plate. Assuming that most of the cells in the original culture were living, how many CFUs do you expect to count on your spread plate the next day? 5) 6) What is the average size of a yeast cell?

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