Why do plasmids used to carry foreign genes into E. coli cells invariably also c

Question

Why do plasmids used to carry foreign genes into E. coli cells invariably also carry the gene for resistance to an antibiotic?
Select one or more:
a. The gene is necessary to safely grow the bacterium in the laboratory without risk of containment breach
b. The gene is required for cellular replication
c. The gene is necessary for the lac operon system to operate
d. To enable selection for only those cells that carry the plasmid with the foreign gene of interest

The activity of pyruvate kinase is controlled by which of the below agents

Select one or more:

a. Protein Kinase A

b. Fructose-1,6-bisphosphate

c. Fructose-6-phosphate

The lac repressor will bind allolactose and IPTG. Can allolactose or IPTG be metabolised?

Explanation / Answer

Answers:-

1:- d) To enable selection for only those cells that carry the plasmid with the foreign gene of interest.

Explanation:- Plasmid vectors contain essential nucleotide sequences required for DNA cloning, replication origin and a drug resistant gene. Cells that contain the plasmid will survive, grow and reproduce in culture containing antibiotics because of drug resistant gene. So those cells can easily select from those cells which do not contain plasmid.

2:- b) Fructose-1,6-biphosphate

Explanation:- The most significant pyruvate kinase regulator is fructose-1,6-biphosphate (FBP) which act as allosteric effector for this enzyme.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.