1. How many grams of Tris do you need to prepare 1L of a 1M stock if the molecul

Question

1. How many grams of Tris do you need to prepare 1L of a 1M stock if the molecular weight of tris is 121.1 g/Mol?
2.how many grams of tris do you need to prepare 100ml of a 1M stock if the molecular weight of tris is 121.1 g/Mol?
3. How many grams of NaCl do you need to prepare 1L of a 5M stock if the molecular weight of tris is 58.4 g/Mol?
4.How many grams of NaCl do you need to prepare 50 ml of a 5M stock if the molecular weight of tris is 58.4 g/Mol?
5. How many grams of sucrose do you need to make 500 ml of a 10% solution?
6. Convert 50mg/ml to ug/ml
7. Convert 50mg/ml to ug/ul
8. Convert the following ( 10mg to ug, 500ml to L, 75mM to M)

Lab math practice problems (Show your work) How many grams of Tris do you need to prepare 1 L of a 1M stock if the molecular weight of Tris is 121.1 g/mol? 1, 2. How many grams of Tris do you need to prepare 100ml of a 1M stock if the molecular weight of Tris is 121.1 g/mol? How many grams of NaCI do you need to prepare 1 L of a 5M stock if the molecular weight of NaCl is 58.4 g/mol? 3. 4. How many grams of NaCI do you need to prepare 50 ml of a 5M stock if the molecular weight of NaCl is 58.4 g/mol? 5, How many grams of sucrose do you need to make 500ml of a 10% solution? 6. Convert 50mg/ml to g/ml 7 Convert 50mg/ml to g41 8. Convert the following 10mg #- 500mlL

Explanation / Answer

1. 1 molar solution means adding 1 mole of a solute to 1 L of water. So, to prepare 1L of 1M tris solution, 121.1 grams of Tris is needed.

2. To prepare 100ml of 1M Tris solution, 12.1 grams of Tris is needed. As to prepare 1000ml 121.1 gms is needed, hence to prepare 100ml = 121.1/10 = 12.1 grams

3. To prepare 1M, 1L solution of NaCl, 58.4 grams needed. To make 5M of 1L solution, 5x58.4 = 292.0 grams is needed.

4. To prepare 50ml of 5M solution the solute needed is 14.6 grams. As, to make 1000ml = 292.0 grams is needed. To make 100ml = 292/10 = 29.2 To make 50ml = 29.2/2 = 14.6 grams.

5. X% = (g sucrose)/(mL solution) x 100 = 10% = (g)/ (500) X100 = 10/100 = g/500 x 100 = 50 gms.

6. 50mg/ml = 50 X 1000 ug/ml = 50,000ug/ml

7.. 50mg/ml = 0.05ug/ul .

8.10 mg = 10 x 1000ug = 10,000 ug

500ml = 500/1000 L = .05L

75mM = 75/1000 M = .075 M

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