A. Indicate whether the following descriptions apply to nutrient, defined, selec
ID: 146706 • Letter: A
Question
A. Indicate whether the following descriptions apply to nutrient, defined, selective,
differential, or enrichment media. If the statement applies to more than one, indicate which ones.
Answers may be used more than once.
____________________ if you want to take an environmental sample and grow a wide variety of
bacteria, you would use this medium
____________________ blood agar is an example, because you can see whether or not microbes
produce substances that lyse red blood cells
____________________ you might make this medium with a specially-formulated salt mixture,
some vitamins, and a refined protein source like peptone
____________________ the lauryl tryptose broth that we used for the presumptive coliform test
is this type of medium- we used it to increase the chance of actually seeing the coliforms
B. Indicate whether the following procedures are best performed with liquid or solid media. If
the statement applies to both, say “both.”
_______________________ you can count colonies of microbes grown with this medium
_______________________ you can grow facultative anaerobes with this medium
_______________________ you can use a spectrophotometer to measure growth in this medium
_______________________ you can prepare this medium by autoclaving it prior to use
Explanation / Answer
A. 1. Nutrient media.
2. Differential media.
3. Nutrient media
4. Selective media. To see the growth of coliforms the other bacterial growth should be inhibited for inhibiting the other growth this selective culture media is used.
B. 1. In both the mediums ( agar medium ) which is solid and liquid. you can count colonies of microbes grown.
2. you can grow faculative anaerobes in solid medium.
3. Spectrophotometer is used to measure the growth in the liquid medium.
4. Solid medium can be prepared by autoclaving. because liquid medium does not need autoclaving for sterillization just a steam heat is enough.
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