Which of the following techniques is suitable for measuring the level of gene tr

Question

Which of the following techniques is suitable for measuring the level of gene transcription in an experimental system? Northern blotting Reverse-transcription polymerase chain reaction (RT-PCR) DNAse I footprinting Western blotting QUESTION 21 Refer to the section on Nucleic acid quantification and any other references. What are the molar extinction coefficients of RNA? QUESTION 22 Which one of the following groups is responsible for absorption of light by nucieic acids at 260 nm? O ribose O deoxyribose 0 phosphates

Explanation / Answer

20- Most sensitive technique is RT-PCR for measuring bevel of gene transcription otherwise northern blotting can also be used to measure level of gene transcription.

21-molar extinction coefficient of RNA is 0.025(microgram/ml)^-1cm^-1 at 260nm wavelength.

22-Bases are responsible for absorption of light at 260 nm in nucleic acid.

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