Scenario #4 our group is on a road trip to San Angelo Texas. eve While down scen

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Scenario #4 our group is on a road trip to San Angelo Texas. eve While down scenic Hwy 206 in Colman County, ryone notice that there is quite a bit of flattened fauna on the road. There was quite a massacre o NT on a small 2-mile stretch of road just outside of Colman. All of you are Biology students at lab. Being sick and twisted and a little on the macabre side, your and work in the molecular biology p stops the car and decides to collect some of the fresh road kill.It just so happens that there are a number of 2ml flip cap microfuge tubes collect in the car. A small piece of tissue from the unknown creature is back in the car and drives down the road to another unrecognizable mass the car and collect that grease spot came from. ed, placed in a tube, caped and place in a cooler of beer in the back of the car. Everyone jumps another sample. The group has a deep seeded need to know what type of creature of flesh on the road, get out of Si.12 si -a You will become familiar with current techniques used in Cell Biology, become familiar with cell re and function, develop protocols to answer research questions, develop critical thinking skills, identification of species utilizing PCR, and process sequencing data utilizing bioinformatics databases. You will receive two samples related to the scenario. You will need to review all of the reading material prepare for lab, Tutorial videos, Background, protocols, instrument manuals, package inserts, etc.). The concept map (PCR and Sequencing of mtDNA) should be used to help guide you through the first project. A scientific notebook is required. The notebook should include a title that clearly describes t Purpose and background information showing why this knowledge is of interest and any literature that is relevant to the project. A testable hypothesis should be clearly stated, not a prediction. Appropriate materials and methods section should be clear enough so that anyone could read and repeat your experiment and have similar results. Data and results section should be in the correct format (table, graph) with no mistakes and shows the relationships between the data without the reader referring to the text. The narration of the results should be very clear and relevant to the hypothesis. Statistical analysis is appropriate, correct and clearly explained. Conclusions should be clearly formed from the stated hypothesis and collected data. Primary literature should be extensively used to make connections between the project and other research in the field. The implications or application of the research should be clearly stated. The notebook should be easily understood due to the excellent grammar, word usage, and organization. You will also be giving a presentation on your work and will be graded on your participation in the group. The Notebook Rubric, Presentation Rubric and Classroom Participation Rubric will be strictly adhered too. There will be a series of short lectures reviewing the instrumentation and its usage prior to you proceeding with your project.

Explanation / Answer

Experiment on raod killed creature.

purpose : road kill creatures are the animals killed by vehicles.it is very important to be aware about their source, what made them come to the roads from their natural habitats.also about their biological structure and changes( if any).to find out out about their extinction level and find out ways to protect them .road kill creatures can be used for several research work as well. they can also be useful for for formation of useful substances for drugs , isolation of certain bacterias etc

expeiments and result on isolation and detection of DNA OR RNA :

BY BOILING METHOD

1)remove cell walls by lyzozyme treatment the do lysis by nonnioc detergent

2)boil for brief period and the cool

3)the chromosomal DNA forms an insoluble clot and denatured protein and cellular debris.

4)the clot is pelleted by centrifugation and plasmid DNA soluble is received as supernatant

5)boiling will FACILITATE THE isolaion ON plasmid DNA IN UNDERGRADED FORM.

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