In bacteria, the level of gene expression is modulated by the degree to which ch

Question

In bacteria, the level of gene expression is modulated by the degree to which chromosomal DNA is underwound. When a highly specific restriction enzyme cleaves the bacterial chromosome at a single site, only the genes within 10,000 bp of the cut site exhibit a change in expression Why is the transcription of the genes more than 10,000 bp away from the cleavage site unaffected by thee strand break? When a restriction enzyme cleaves the bacterial DNA at a single site, the DNA outside of the 10,000 bp range is overwound to compensate for the DNA relaxation within 10,000 bp of the cut site. Topoisomerase I is bound to the bacterial chromosome at approximately 10,000 bp intervals. When a highly specific restriction enzyme cleaves the DNA, topoisomerase l restricts the DNA relaxation to within 10,000 bp of the cut site O e restriction enzyme cleaves the bacterial chromosome during prophase, when the DNA is highly condensed. Therefore, cleavage at a single site relaxes only a small portion of the chromosome, equal to approximately 10,000 bp The bacterial chromosome is organized into nucleosomes that are approximatel O 10,000 bp long. Histone H1 at the boundaries of each nucleosome restricts the DNA relaxation to within 10,000 bp of the cut site Each domain in the bacterial nucleoid is approximately 10,000 bp long and topologically constrained. Therefore, cleavage by a restriction enzyme at a single site can only relax the DNA within a domain

Explanation / Answer

I believe the best possible explaination could be:

E) Each domain in the bacterial nucleoid is approximately 1000bp long and topologically....

all the rest seems inaccurate.

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