L) Which one of the following statements is not true regarding gel filtration ch
ID: 146923 • Letter: L
Question
L) Which one of the following statements is not true regarding gel filtration chromatography? Larger molecules elute first during a gel filtration chromatography experiment. Washing of a gel filtration column with high salt concentrations is usually required to break up interactions between sample proteins and the column resin Large molecules are excluded from the pores of the gel filtration resin (material). Gel filtration chromatography separates molecules based on size. 2) Fill in the blanks: A protein with many more E and D residues than K and R residues would be charged at physiological pH, and could likely be purified using chromatography negatively, cation exchange negatively, anion exhange positively, cation exchange positively, anion exchange What is the estimated size of the protein indicated with the arrow in its native state if it is a homotrimer (i.e. at what size would it run on a gel filtration column under non-denaturing conditions)? 1 MW 70 50 35 28 15 195 kDa cannot tell from the given information -130 kDa -65 kDaExplanation / Answer
1) In Gel filtration chromatography the second statement is false.
B) washing of gel column with high salt concentration is not required
A) Larger molecules elute faster in gel filtration chromatography experiment. Because in gel filtration chromatography large molecules cannot enter into gel and are excluded they have less volume to transvers and elute soonar.
C&D) In gel filtration chromatography the separation of proteins based on the size of the molecule.
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