Phe c True or False. Determine the following statement is true or false by indic

Question

Phe c True or False. Determine the following statement is true or false by indicate a "T" or "F", respectively in the brakets. (1 point/question, 5 points in to B. tal) a. All mutations cause genotypic changes.) b. Antibiotic resistant variants can emerge even in absence of any antibiotic selection pressure. ) c. For selection of aunoph muants by penii ichment just one eycle of enrichment is sufficient for having about 10% cells with auxotrophy. ( d. Replica plating can be employed for selecting auxotrophic cells.) e. Point mutations are in general non-revertible.

Explanation / Answer

a) False;

mutations occuring in sex chromosomes bring about genotypic changes, but somatic mutations are not a part of an individuals genotype.

b) True;

Antibiotic Resistance variants multiply and grow in the presence of antibiotics and due to their adaptation to the changes,they can emerge/grow in their absence as well. Once adapted the trait cannot disappear, moreover it will be disadvantageous for their evolutionary fitness.

c)False;

Penicillin enrichment method is used to isolate mutants. Penicillin kill most but not all of the cells that grow, and enriches the mutants. Since auxotrophs are rare in a population of cells, getting 10% of the mutations in one cycle is not possible.

d) True;

The replica-plating technique is used to identify mutants. Auxotrophic mutant require different nutrient growth media than the wild type. The microbes that do not grow on the minimal medium are the auxotrophic strains.

e) False;

Point mutations are revertible, they can be reversed by reverting the sequence.

deletion mutations are non-revertible.

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