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The following mRNA encodes for a virulence factor in (the bacterium that cause t

ID: 14716 • Letter: T

Question

The following mRNA encodes for a virulence factor in (the bacterium that cause the bubonic plague)It allows this pathogen to live in environments with low [Ca2+]. Notice that the Shine sequence is underlined. What is the amino acid sequence of the portion of the protein encoded by the mRNA shown her? Regarding the prokaryotic ribosome The 23 S rRNA within the 50S subunit catalyzes the formation of peptide bonds during the elongation phase of translation. The 16S rRNA within the 30S subunit contains a pyrimidine-rich sequence that base pairs with the Shine sequence. The large subunit is the last thing to join the initiation complex before the elongation phase of translation begins. The E-site is the site through which a tRNA leaves after the translocation phase of elongation. All of the above. The picture below is a cartoon depicting the elongation phase of translation. Which statement/s is/are TRUE? [*Please mark all that apply!] The amino acid bound to the tRNA in

Explanation / Answer

1. From the given mRNA, the protein would be: The correct answer is B. There are two AUG codons in the sequence. As the first is part of the Shine Dalgarno sequence which binds to the 16S rRNA, it wont be a part of the protein. The second AUG codon is 5 basepairs after the Shine Dalgarno sequence. Hence the translation of the protein starts from the second AUG codon. ------------------------- 2. Regarding the prokaryotic ribosome The correct answer is E- all of the above. All the given statements of a-d are correct. --------------------------- 3. In the given picture: The correct answers which apply are a,d. The other options are wrong. The next step will not be translocation but formation of peptide bond. The tRNA in the A site will not be the next tRNA to leave the ribosome but the tRNA in the P site as the ribosome will translocate a distance of 3 bases which will move the tRNA in P site to E site and the tRNA from A site to P site.