8. Two codons (CAG and CAA) are used for the amino acid glutamine. (10 points in

Question

8. Two codons (CAG and CAA) are used for the amino acid glutamine. (10 points in total) What is the minimal number of tRNA-Gln nceded to read these codons? List the anticodon(s) expected for each predicted tRNA-Gin. (3 points) a. Ankcodons O For CAG:GUC b. An amber suppressor (sup-2) is due to a mutation in a glutamine tRNA gene suppressor tRNA to have? (2 points) What anticodon would you expect this AU C An ochre suppressor (supE) is also due to a mutation in a glutamine tRNA gene. What anticodon would you expect this suppressor tRNA to have? (2 points) c. CUA A double-mutant carrying both suppressor mutations (sup-2 and supE) can be made. The double-mutant suppresses both the amber and ochre mutations and d.

Explanation / Answer

A) Minimum one tRNA-Gln is needed to read these codons. The anticodons to read these codons are :

3'-GUU-5' for 5'-CAA-3', 5'-CAG-3' and

3'-GUC-5' for 5'-CAG-3'.

B) The suppressor tRNA will have the anticodon anything except

3'-GUC-5', 3'-CUA-5' and 3'-UUA-5'.

C) The suppressor tRNA will have the anticodon anything except 3'-UUA-5', 3'-UUG-5'.

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