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Wecome MicrsoMere Mal pani l.edu/bbcswebdav/pid-3823350-dt-content-rid-2193 Ourses/1061-201 8FA/Quiz%205%281%29.pdf Quiz 5 You are given two haploid strains of yeast with opposite mating types. Both of these strains grow on minimal medium containing arginine, but not on minimal medium lacking arginine. You generate a diploid strain by mating them. The diploid grows on minimal medium lacking arginine, suggesting that the two strains have recessive mutations in different genes, both of which cause an Arg-growth phenotype. We will designate them arg20 and arg30. You induce diploid cells to undergo meiosis (sporulation) and analyze 100 of the resulting tetrads for ability of the haploid spores to grow in the absence of arginine. You find three different classes of tetrads, in the numbers indicated below Arg : Arg 4 :0 40 20 40 2: 2 a) Are arg20 and arg30 linked? (yes or no) b) Can you say anything else about the chromosomal locations of these genes, in three sentences or fewer?

Explanation / Answer

A) Yes, these are linked genes because they tend to stay together during the formation of gametes. Thus the results of test cross and self cross involving two genes yield different results.

B) The diploid zygote proceeded through meiosis to produce four haploid cells, which are called spores. This group of four spores is known as a tetrad.If meiosis followed by a mitotic division to produce eight cells, known as an octad.

The arrangement of spores varies from species to species. If octad mix randomly, it is called as unordered tetrad or octad. These produce a tight arrangement that prevents spores from randomly moving around. This can create a linear tetrad or octad.

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