What reaction is being catalyzed in this experiment? What is the enzyme in this

Question

What reaction is being catalyzed in this experiment?

What is the enzyme in this experiment? What is the substrate?

What is the independent variable in this experiment? What is the dependent variable?

How does the temperature affect enzyme function? Use evidence from your data to support your answer.

Draw a graph of balloon diameter vs. temperature. What is the correlation?

Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.

In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.

Design an experiment to determine the optimal temperature for enzyme function, complete with controls.

Where would you find the enzymes for this experiment? What substrate would you use?

test tube balloon circumference cm ballon final circumference cm refrigator room temp Hot water

Explanation / Answer

test tube

balloon circumference cm

ballon final circumference cm

refrigator

4.5 inches

7 inches

room temp

4.5 inches

7.5 inches

Hot water

4.5 inches

9 inches

What reaction is being catalyzed in this experiment? Production of oxygen gas

What is the enzyme in this experiment? What is the substrate? Yeast, the substrate is the hydrogen peroxide and the balloon was the source of the enzyme in this experiment because the yeast was intering the substrate from the balloon.

What is the independent variable in this experiment? What is the dependent variable? The independent variable is the yeast added to the hydrogen peroxide and the dependent variable is how much oxygen gas is produced.

How does the temperature affect enzyme function? Use evidence from your data to support your answer. The colder test tube was smaller; 7 inches compared to the 7.5 inches of the room temperature balloon; therefore less oxygen was produced and also less heat was produced, although some heat was still produced.

test tube

balloon circumference cm

ballon final circumference cm

refrigator

4.5 inches

7 inches

room temp

4.5 inches

7.5 inches

Hot water

4.5 inches

9 inches

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