1. (4 points) Given that the average weight ofan amino acid is i 10 daltons, det
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1. (4 points) Given that the average weight ofan amino acid is i 10 daltons, determine the following. Remember to convert between daltons and kilodaltons. Show your work for full credit. A. An actin monomer is 42 kD. Approximately how many amino acids does it contain? I omino ood l L IO D X-A3,000D x- 3818 amme B. Protein C contains 532 amino acids. What is its size in daltons? 1 amino ou 58, So 2. (6 points) In a brief paragraph, explain how solubilized proteins were obtained from the fish muscle samples when you prepared the protein extracts. What is the purpose of the homogenization buffer, vortexing and centrifugation steps (what effect do they have on the cells/extract)? Be sure to explain the purpose of all three for full credit. Centi fuguion tu centa debi ot Tu tsn o tu outorn win it was H The extrachin ot Page 1 of 3Explanation / Answer
an actin monomer is 42kda and average weight of an amino acid is 110DA
110 Da= 1 amino acid
1 Da= 1/110 amino acid
42000=1/110 ×42000
=381.8 amino acids
Protein C contain 532 amino acids
The weight of protein 532×110
=58520Da
58.52 Kda
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