PLEASE SOMEONE HELP!!! can someone please interpret these images for me. This is

Question

PLEASE SOMEONE HELP!!! can someone please interpret these images for me. This is my first paper and I'm nervous, so just want to make sure I interpret the images correctly it's immunostaining of larval neuromuscular junction and motor neurons. First picture is HRP stain, second GFP stain. if anyone can place arrows and just explain the images I would greatly appreciate it. Like what are the bright spots indicating? Can you see where the NMJ and motor neurons are?

doesn't need arrows, but I just would like explained what the images show so I can write a correct assessment. Thank you to whoever can help me

Tagged molecule of GFP is Green: muscle maker (phalloidin), substrate of HRP is Red: synapse maker (Dlg) Anti-HRP-Cy5

Explanation / Answer

This study has done for the study of synaptic development and plasticity. Third instar larval muscle fillets are dissected and double-labeled with an anti-HRP antibody to visualize the neuronal membrane and an anti-Dlg antibody to mark the subsynaptic reticulum. The expression of a membrane GFP was used to mark the presynaptic vesicles of the motor neurons (phalloidin staining : detects F-actin, at the postsynaptic bouton). Usually, HRP antibodies that recognize the neuronal membrane and/or the pre/postsynapse (e.g., DLG).

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