If a culture containing 3,345,987,110 cfu/mL is diluted 1000 times (1000-fold),

Question

If a culture containing 3,345,987,110 cfu/mL is diluted 1000 times (1000-fold), what is the concentration of cells in the diluted culture? Please write your answer in scientific notation. 10. If a culture containing 3.35 x 109 cfu/mL is diluted 10 times, what is the concentration of cells in the diluted culture? Please write your answer in scientific notation. 11. If a 103 dilution is prepared from a culture containing 3.35 x 10° cfu/mL, what is the concentration of cells in the diluted culture? Please write your answer in scientific notation

Explanation / Answer

9) If a culture containing 3.34 X 109 CFU/mL, and diluted 103 times then the concentration of cells in the diluted culture is 3.34 X 106 CFU/mL.

10) If a culture containing 3.35 X 109 CFU/mL, and diluted 103 times then the concentration of cells in the diluted culture is 3.35 X 106 CFU/mL.

11) If a culture containing 3.34 X 109 CFU/mL, and diluted 10-3 times then the concentration of cells in the diluted culture is 3.34 X 1012 CFU/mL.

13) A) Bottle Total dilution factor

1 102

2 104

3 105

B) Bottle Volume plated Number of colonies

1 0.1 ml   2.45 X 106

1 ml 2.45 X 107

2 0.1 ml   2.45 X 104

1 ml 2.45 X 105

3 0.1 ml   2.45 X 103

1 ml 2.45 X 104   

C) No, there will be different number of colonies will be developed. Because inoculum for each plate were spread on solid plates. If they mixed and poured, the culture solution becomes homogenized and the number of colonies will be differed.

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