number 1 please (tabulate all data needed in determining monod parameter) and ho

ID: 148332 • Letter: N

Question

number 1 please (tabulate all data needed in determining monod parameter)
and how to determine each parameter (the equation)

An experiment was done to determine the maximum growth rate (Hma) and saturation constant ( Ks) of the growth of Escherichia coli. A series of flasks containing fresh and sterile broth are prepared, different initial substrate concentration (So) was introduced to each flask. The same E. coli concentration was introduced with xo 0.3 g/L. The flasks were then incubated for 30 minutes. After incubation the products (P) and substrates (S) concentration were determined, while the cell concentration is measured by using spectroscopy method and stated as absorbance (A). The cell concentration (A) is measured after the cell concentration was diluted with dilution factor of 5. The experimental data obtained is as follows: Flask 0.3201 0.3336 0.3489 0.3662 0.3858 0.3943 0.4033 0.4278 0.4556 0.4616 0.4677 So (8/L) 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5 0.55 0.6 P (g/L) 0.085 0.127 0.169 0.200 0.232 0.266 0.3 0.3 0.3 0.3 0.3 4 6 10 The amount of product formed is equal to the amount of substrate consumed during incubation time period. A standard curve describing the relation between absorbance and cell concentration is prepared as follows A (a.u. 0.2601 0.3102 0.4281 0.5211 0.6012 0.7002 x (g/L) 0.05 0.1 0.2 0.3 0.4 0.5 From the obtained data, answer the following questions 1) Tabulate all data needed in determining Monod parameters. 2) Draw the non-linearized Monod graph and determine the Monod parameters 3) Draw the reciprocal graph of Monod and determine the Monod parameters.

Explanation / Answer

Calibration curve b/w absorbance and cell conc. gave equation:- y= 1.58* X

y= (absorbance), X= (cell conc.)

calculated biomass conc. (X)

specific growth rate(u)= 1/x*dx/dt

Table for determining monod parameteres

Flask no

1/X

1/S

dx/dt

1.012975

0.987192

0.015

66.66667

0.023766

0.023462

42.7

1.055696

0.947242

0.023

43.47826

0.02519

0.023861

42.01

1.104114

0.905704

0.031

32.25806

0.026804

0.024276

41.322

1.158861

0.862916

0.05

0.028629

0.024704

40.48

1.220886

0.819077

0.07

14.28571

0.030696

0.025142

39.84

1.247785

0.80142

0.084

11.90476

0.031593

0.025319

39.51

1.276266

0.783536

0.10

0.032542

0.025498

39.37

1.353797

0.738663

0.15

6.666667

0.035127

0.025947

38.61

1.441772

0.693591

0.2

0.038059

0.026397

38.022

1.460759

0.684575

0.25

0.038692

0.026488

37.87

1.480063

0.675647

0.3

3.333333

0.039335

0.026577

37.73