In my Biology lab we have: undiluted 10x 100x ...diluted DNA samples It says to
ID: 1484 • Letter: I
Question
In my Biology lab we have: undiluted 10x 100x ...diluted DNA samples It says to measure the A260 of each DNA samplestarting with the 100X first. It says that if the absorbance of this is <0.2 than Ishould measure the A260 of the 10X or 1X I don't understand!! Also...what if it is more than 0.2absorbancy? What would that mean???? In my Biology lab we have: undiluted 10x 100x ...diluted DNA samples It says to measure the A260 of each DNA samplestarting with the 100X first. It says that if the absorbance of this is <0.2 than Ishould measure the A260 of the 10X or 1X I don't understand!! Also...what if it is more than 0.2absorbancy? What would that mean???? undiluted 10x 100x ...diluted DNA samples It says to measure the A260 of each DNA samplestarting with the 100X first. It says that if the absorbance of this is <0.2 than Ishould measure the A260 of the 10X or 1X I don't understand!! Also...what if it is more than 0.2absorbancy? What would that mean????Explanation / Answer
In general, the reason for measuring 100x first is because you'resupposed to measure the A260 by lowestconcentration first. This way, if your absorbance is over 0.2, thenyou can stop measuring and record your data. If it's under 0.2, you might want to continue on and check theother A260 to find which one hits over. Hope this helps. ADDITIONAL: Ahh, you would like to know what happens if you measurethe 10x or undiluted, considering the 100x was lower than 0.2? Well, you want an absorabancy of over 0.2. So let's say youdid the 100x and got 0.01, then you did the 10x and got 0.1. Thegoal is to get over 0.2, which would give you, let's say, the "mostaccurate result", so if undiluted gave you 0.2, then you would begood to go. You start from the most diluted, and go to the most undiluted.Whenever you hit 0.2 or more, you would stop and use that as yourdata. Please feel free to ask me again if this isn't enough. ADDITONAL: I believe I misread your question. Your data, if you continued to gather it, would prove lessaccurate. The LESS you go over 0.2, the more accurate your dilutionconcentration is. Does that help?
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