Suppose that researchers discover a new organism with a novel 3\' to 5\' DNA pol

Question

Suppose that researchers discover a new organism with a novel 3' to 5' DNA polymerase and a novel dNTP that allows DNA replication without the requirement for a lagging strand. The organism can replicate the antiparallel strands of duplex DNA using both a typical 5 to 3' polymerase and this novel 3 to 5 polymerase. Choose a plausible mechanism of DNA synthesis for this novel DNA polymerase and novel dNTP The nucleophilic attack of the existing strand 3'-OH on the a phosphate of a nucleotide 5'-triphosphate. The nucleophilic attack of the existing strand 5'-OH on the phosphate of a nucleotide 3'-triphosphate The nucleophilic attack of the existing strand 3-OH on the phosphate of a nucleotide 5'-triphosphate. The nucleophilic attack of the existing strand 3'-0H on the a phosphate of a nucleotide 3'-triphosphate. The nucleophilic attack of the existing strand 5'-OH on the phosphate of a nucleotide 3'-triphosphate.

Explanation / Answer

Answer

Option (B) the Nucleophilic attack of the existing strand 5' -OH on the alpha phosphate of a nucleotide 3' -triphosphate

in the presence of novel 3'-5' polymerase, the free 5'OH group of novel nucleotide of the newly synthesizing chain (over the antiparallel strand ) will make a nucleophilic attack on the alpha-phosphate of incoming novel 3'dNTP, causing phosphodiester bond formation in 3'-5' direction and releaseing PPi.

The reaction will then continue with the free 5'OH group of this newly added nucleotide.

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