Do lipids mix with water? Why/why not? From your research on each of these reage
ID: 148886 • Letter: D
Question
Do lipids mix with water? Why/why not? From your research on each of these reagents, and reading your lab,fill in the following tables with your predicted outcomes for each macromolecule test you will be performing. Table 1. PREDICTED results for tests for monosaccharides, reducing sugars &complex carbohydrates (note as + or-and the resulting color) Reducing sugar Monosaccharide Starch Carbohydrates Reagent used for testing: Glucose Fructose Lactose Sucrose Starch (example below + orange C&H1206 C&H1206 C12H2201 C12H201 Glycogen CsHioOs Table 2. PREDICTED results for protein identification using ninhydrin Contents Distilled water clear ColourWhy? (explanation-what is each substrate?... Test tube # because H:0 has no amino acids in it (negative control) Gelatin Albumin Unknown C Table 3. Predicted results for Biurets test and why Tube #Vol. of sample Vol dH0 Vol. Predicted outcome and why Biurets 0 ml 1 ml 1ml Negative control 20.5 ml ptone 05 ml ml l ml 4 1 ml gelatinmml 5 1.0ml unkown D 0ml1 ml Table 4. Predicted results of testing molecules for the presence of DNA Test tube # Contents Reaction with Dischedphenylamine why? explanation) dH20 1 ml DNA 0.5 ml DNA & H20 1 ml RNA ON NEXT PAGE: Blank macromolecules chart to be filled in for study purposes.Explanation / Answer
Answer (Table 2):
Test tube #
Contents
Color
Explanation
1
Distilled Water
Clear
Because water has no amino acid
2
Glycine
Dark violet
Confirms the presence of amino acid
3
Gelatin
Blue violet
Confirms the presence of amino acid
4
Albumin
Indigo
Confirms the presence of amino acid
5
Unknown C
NA
NA
Explanation:
Ninhydrin Test is for detecting the existence of amino acids. Ninhydrin is an oxidating agent that leads to deamination of alpha-amino acids and proteins that contain free amino acids. It degrades amino acids into aldehydes, ammonia, and carbon dioxide. The net result is a reduced form of hydrindantin. When ninhydrin condenses with ammonia, hydrindantin produce a bluish-purple pigment known as the Rhuemann’s purple.
Test tube #
Contents
Color
Explanation
1
Distilled Water
Clear
Because water has no amino acid
2
Glycine
Dark violet
Confirms the presence of amino acid
3
Gelatin
Blue violet
Confirms the presence of amino acid
4
Albumin
Indigo
Confirms the presence of amino acid
5
Unknown C
NA
NA
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