Consider the “immunity of a lysogen to a phage: a) Explain why a wild-type phage

Question

Consider the “immunity of a lysogen to a phage: a) Explain why a wild-type phage cannot grow in a lysogen. b) Will a cl- mutant strain of phage grow in a lysogen? Why or why not? c) Mutations that allow to grow in a lysogen are called virulent mutations (vir). What part of would you mutate to create this strain, and can you relate this type of mutation to previous operon mutations you have encountered in your studies? Consider the “immunity of a lysogen to a phage: a) Explain why a wild-type phage cannot grow in a lysogen. b) Will a cl- mutant strain of phage grow in a lysogen? Why or why not? c) Mutations that allow to grow in a lysogen are called virulent mutations (vir). What part of would you mutate to create this strain, and can you relate this type of mutation to previous operon mutations you have encountered in your studies? Consider the “immunity of a lysogen to a phage: a) Explain why a wild-type phage cannot grow in a lysogen. b) Will a cl- mutant strain of phage grow in a lysogen? Why or why not? c) Mutations that allow to grow in a lysogen are called virulent mutations (vir). What part of would you mutate to create this strain, and can you relate this type of mutation to previous operon mutations you have encountered in your studies?

Explanation / Answer

a. This is because wild type of lambda have repressor proteins which are suppressed by Lamda phage.

b. CI mutant suppressed the expression of regulatory proteins in phage. Hence, cl- strain allow the lambda phage grow in lambda lysogen.

c. The mutations that are responsible for converting virulence strain are mutation in the operator and repressor sequence.Yes .this mutation is related with mutation in cl- mutant strain.

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