The second video will be of a plasma membrane protein called VSVG fused to GFP (

Question

The second video will be of a plasma membrane protein called VSVG fused to GFP (green fluorescent protein). Consider the following questions: 1. In which compartment do plasma membrane proteins begin their journey? 2. What should the fluorescence pattern of a protein in that compartment look like? 3. What is the next destination of that protein? 4. What should the fluorescence pattern of that compartment look like with the fluorescent protein? 5. What should happen to the fluorescent signal in that compartment over time (as the protein moves on to the plasma membrane)?

Explanation / Answer

1. Protiens destined to be secreted move through the secretory pathway in the following order:

Rough ER to ER-to-Golgi transpot vesicles to Golgi cisternae to secretory or transport vesicles to Cell surface (exocytosis)

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