BI 420L Medical Microbiolo Fixing a Slide and the Gram Stain 10 points In the fo
ID: 149062 • Letter: B
Question
BI 420L Medical Microbiolo Fixing a Slide and the Gram Stain 10 points In the following vid and the Gram stain: eo you can view the procedure for fixing a slide https://youtu.be/sxa46xKIOY Answer the following questions: 1. What color would Gram negative bacteria be after the first step, i.e. staining with crystal violet? 2. Why did Hans Christian Gram develop the stain, i.e. what was his original purpose for the stain? 3. How does Streptococcus pyogenes appear after negative? What color are they after the stain? 4. How does Salmonella typhi appear after staining the Gram stain, i.e. Gram positive or Gram with the Gram stain? What color are they after the stain? 5. Explain why we think bacteria stain differently with the Gram stain. In other words, how do Gram positive and Gram negative bacteria differ?Explanation / Answer
1. Gram stain bacteria will have pink color after staining as it have thin layer of peptidoglycan through which violet color enter the cell.
2. Hans Christian gram developed originally staining to see the bacteria clearly under the microscope.
3. Streptococcus pyrogen is a gram positive bacteria. Hence, it will have purple color after staining.
4. Salmonella typhii is a gram negative bacteria .Hence, it will have pink color.
5. Gram positive and gram negative bacteria differ in the cell wall composition. Gram positive bacteria have thick peptidoglycan layer whereas gram negative bacteria have thin peptidoglycan layer.
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