Introduction ng experiments each group will examine the effects of temperature,
ID: 149157 • Letter: I
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Introduction ng experiments each group will examine the effects of temperature, inhibition, enzyme concentration, specificity, time or pH on the activity of lactase. Names of Group members: Role Assignment: Coordinator Materials manager: Experimentalist:Shndon Recorder: Exercise 2: The Effects of Temperature on Enzyme Activity n this experiment, you will determine how temperature effects optimal enzyme activity Each enzymatic reaction has an optimum set of conditions that produces the most efficient enzymatic activity (faste enzymes and with the location of the reaction in the body. Many enzymes also require the presence of inorganic or organic enzyme helpers (cofactors and coenzymes) in order to function properly. Other factors that affect enzyme activity include: temperature, pH, the concentration of substrates, and the concentration of enzymes. Enzyme inhibitors (see Figure 4.3) can also affect the binding of substrates by causing the active site to undergo a conformational change preventing substrate binding. When the three-dimensional shape of the enzyme is disrupted, the protein is said to be denatured and the enzyme is inactivated. st reaction rate). Optimum conditions may vary with different Procedure (Group 1) 1. Generate a hypothesis concerning the impact of temperature on enzyme activity Hypothesis: 2. The instructor will set the baths to 40°C 60- 80 °C Check the thermometers in the water baths and adjust the temperature knobs slightly, if needed, to achieve the correct temperatures. 3. Gather five 1.5 ml microcentrifuge tubes and label them as O°C lice bucket), 25°C (room temperature), 40 eC, 60 °C, 80 °C. 4. Add 10 drops of skim milk to each tube. 5. Add 10 drops of lactase to each tube. 6. Close the lids and invert the tubes two to three times to mix the solutions. 7. Place tube marked as 0° C in the ice bucket. Laboratory 4Enzymes 73Explanation / Answer
A hypothesis is a supposition. We seek experimental evidence to prove or disprove a hypothesis.
Since the experiment examines the effect of temperature on the enzyme lactase, the hypothesis will also be related to temperature.
Some options to choose the hypothesis from are:
1. Lactase has optimum activity at temperatures below 50 degrees
2. Lactase gets denatured at temperatures above 50 degrees.
3. Lactase activity increases with increase in temperature
4. Lactase activity is unaffected by changes in temperature in the range 0-80 degrees.
Lactase converts lactose into glucose and galactose. (Hence the sweet taste of milk)
In this experiment, the activity of lactase was measured at temperature intervals ranging from 0-80 degrees. Equal amounts of milk and lactase were mixed and the amount of product (glucose) was measured. The following reaction occurred.
This happened in the experiment: Lactose (milk) --------> Glucose + Galactose
The amount of lactase in 10 drops can yield 100 mg/dl of glucose at all temperatures from 0-80 degrees.
Interpretation: The results show that the lactase activity is constant and does not vary with temperature.
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