I need help with numbers 1, 2, and 4. 1. What makes the reflective tissue of the

Question

I need help with numbers 1, 2, and 4.

1. What makes the reflective tissue of the Hawalian bobtail squid, Euprymna 2. Is the isolation of reflectins a fairly complex or simple procedure? Please scolopes, unique among reflective tissues throughout the animal kingdom? justify your answer by referring to a particular figure or protocol from the paper 3. Why are there so many bands in the left half of figure 2C and so few bands in the right half? Please include in your answer a brief description of the technique used to produce the figure Would an amino acid composition of reflectins be a valuable piece of information? Why or why not? Is the amino acid sequence less, equally, or more valuable than the amino acid composition? Again, refer to a specific figure and the techniques used to generate that figure as part of your answer. 4.

Explanation / Answer

1. The bobtail squid has developed specialized proteins in its belly that reflect light given off by luminescent bacteria that live on the squid. A minimum of six genes in 3 subfamilies encode the specialized proteins known as reflectins. The unique feature lies in the fact that the genes coding reflectins have not been reported as homologs outside of the squid community. The members of the squid family have highly conserved five repeating domains.

2. Isolation of reflectins is a fairly complex procedure. A proper homogenization of the light organ reflector is essential for extraction of reflectins. Further, the supernatant of the SDS-solubilized pellet which comprises 40% of the proteinaceous component needs to be collected properly for obtaining the proteins. also relectinns make about 50% of the total protein , therefore the procedure must be optimised enough to get a good amount of reflectin proteins to easily back calculate the amount in whole homogenate.

4. Reflectin amino acid composition is rich in methionine, glycine, and arginine and aromatic amino acids tyrosine and tryptophan while lacking the hydrophobic amino acids valine, alanine, leucine, and isoleucine. Therefore, the unique amino acid composition is a valuable piece of information. However, unlike the other reflectins, it has a unique tandem repeat sequence. This makes it different from other squids. Hence, the amino acid sequence gives more valuable information than the amino acid composition.

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