How does salt (sodium chloride) affect the attachment between histone proteins a

Question

How does salt (sodium chloride) affect the attachment between histone proteins and DNA in experiments such as that of Fritz Thoma? Salt decomposes the DNA into individual nucleotides even at low concentrations, thus fragmenting the nucleosomes apart. O Salt ions only affect histone proteins and DNA if the cell is from a freshwater organism like a paramecium O The salt ions strengthen the binding of histones to DNA o At higher concentrations, salt ions block the attraction between negatively charged DNA and positively charged histone proteins.

Explanation / Answer

ANSWER-)

At higher concentrations salt ions block the attraction between negatively charged DNA and positively charged histone proteins.

Binding of Histone proteins to DNA is essential to the formation of nucleosome complex and chromatin structure. H2A, H2B, H3 and H4 are the core histone proteins. Histones are positively charged and water soluble.

Higher salt concentrations can remove the linking of DNA to histones because these are weak interactions. Higher salt concentration is known to cause the precipitation of proteins a phenomena called “Salting-out”.

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