Here you have the non-template strand of DNA sequences of genomic DNA and mRNA o

Question

Here you have the non-template strand of DNA sequences of genomic DNA and mRNA of the human insulin gene (1431nt long from +1nt). The underlined sequences represent the exons and the non-underlined sequences represent introns. The represents number of nucleotides (nt) 3. .CAAT.60ntTATA... .AGCCCTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTGTTCCAAGG GCCTTTGCGTCAGGTGGGCTCAGGATTCCAGGGTGGCTGGACCCCAGGCCCCAGCTCTGCAGCAGGGAGGACGTGGCTGGGC TCGTGAAGCATGTGGGGGTGAGCCCAGGGGCCCCAAGGCAGGGCACCTGGCCTTCAGCCTGCCTCAGCCCTGCCTGTCTCCCA GATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCC GCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGA GGCTICTICTAC ACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGGTGAGCCAACTGCCCATTGCTGCCCCTGGCCGCCCCCAGCCACCCC CTGCTCCTGGCGCTCCCACCCAGCATGGGCAGAAGGGGGCAGGAGGCTGCCACCCAGCAGGGGGTCAGGTGCACTTTTTTAAA AAGAAGTTCTCTTGGTCACGTCCTAAAAGTGACCAGCTCCCTGTGGCCCAGTCAGAATCTCAGCCTGAGGA GGCAGCCCCGAGATACATCAGAGGGTGGGCACGCTCCTCCCTCCACTCGCCCCTCAAACAAATGCCO CCTCATTGATGA CTCTGGGCGAACACCCCATCACGCCCGGAGGAGGGCGTG GCAGCTCCATAGTCAGGAGATGGGGAAGATGCTGGGGACAGGCCCTGGGGAGAAGTACTGGGATCACCTGTTCAGGCTCCCAC TGTGACGCTGCCCCGGGGCGGGGGAAGGAGGTGGGACATGTGGGCGTTGGGGCCTGTAGGTCCACACCCAGTGTGGGTGACC CTCCCTCTAACCTGGGTCCAGCCCGGCTGGAGATGGGTGGGAGTGCGACCTAGGGCTGGCGGGCAGGCGGGCACTGTGTCTCC CTGACTGTGTCCTCCTGTGTCCCTCTGCCTCGCCGCTGTTCCGGAACCTGCTCTGCGCGGCACGTCCTGGCAGIGGGGCAGGTGG AGCIGGGCGGGGGCCCTGGIGCAGGCAGCCTGCAGCCCTIGGCCCTGGAGGGGICCCTGCAGAAGCGTGGCATIGTGGAACA ATGCIGTACCAGCATCIGCTCCCTCTACCAGCIGGAGAACTACIGCAACTAGACGCAGCCCGCAGGCAGCCCCACACCCGCCGC CTCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAGC CGGTGTTGGCTTC CGCAGCCCATTTCTCCAC CCGCAGATTCAAGTGTTTTGTTAAGTAAAGTCCTGGGTGACCTGGGGTCACAGGGTGCCCCACGCTGCCTGO GCTGCCTGCCTGAGTGGGCCAGACCCCTGTCGCCAGGCCTCACG Label the following on the DNA strand above: 1. +1 position, upstream and downstream sequence relative to +1 nucleotide 2. Promoter region 3. Exons, introns, and splicing signals (dinucleotides) Label the following on the mRNA strand below: 1. Start, stop codons, and Kozak sequence

Explanation / Answer

Q3.

1. + 1 position = A base of ATG represented in bold

Sequence left side of bold ATG is upstream and sequence right side of bold ATG is downstream

sequence.

2. Promoter region

-----CAAT-----<60nts>-------TATA------<28nts>-----

3. underlined sequences are exons and in detween these undelined sequences are introns which are not underlined. 5` dinucleotide (GT) of introns and 3` dinucleotide (AG) of introns .

Labeling mRNA

1. Start codon = Bold AUG , stop codon = Bold UAG, kozak sequence = GCCAUGG

Q4.

The insulin protien formed in the bacterial system would not be functional because there is no splicing machinary in the bacteria so introns are not removed.

  

  

  

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