Exercise 1 Transcription, Translation (6 pts) The following is a partial DNA cod

Question

Exercise 1 Transcription, Translation (6 pts) The following is a partial DNA coding segment from Homo sapiens CD4 gene that encodes a membrane glycoprotein of Tlymphocytes. Note that it is not the template strand that RNA was transcribed based on See notes of Chapter2, the figure on page 17, Transcription, for details. 5'ACCGGGGAGTCCCTTTTAGGCACTTGCTTCTGG3' 1. (3 marks) Produce the corresponding RNA fragment. 2. (3 marks) Produce the partial primary protein sequence (one letter codes for amino acids) using reading frame 3. Note: Reading frame 3 means the transcription starts at the 3rd nucleotide base.

Explanation / Answer

1. As transcription is occurring 5' to 3' direction the RNA sequence will be

5' ACCGGGGAGUCCCUUUUAGGCACUUGCUUCUGG 3'

2. if transcription starts with 3rd nucleotide base then the RNA sequence will be

5' CGG GGA GUC CCU UUU AGG CAC UUG GUU CUG G 3'

And the peptide sequence will be

- R-G-V-P-F-R-H-L-V-L-

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