Problem Set 3 Name: 1. (Chapter 10) Place the chromosome packaging structures be

Question

Problem Set 3 Name: 1. (Chapter 10) Place the chromosome packaging structures below (some still hypothetical) in the proper order from lowest to highest structural level. Protein filament scaffold 30-nm chromatin fiber Histone protein Radial loop domain Histone core 2. (Chapter 11) Assume that a eukaryotic cell suffers a mutation that prevents its proofreading capability in a DNA polymerase from functioning properly until after it attaches the first 20 or so nucleotides to the primer sequence laid down by DNA polymerase a. For which of the polymerases 8 or e would the mutation be most harmful? Why? (Keep in mind that this question is NOT about the epsilon subcomponent of prokaryotic DNA polymerases)

Explanation / Answer

1) Chromatin is arranged in a complex structure in which DNA combines with Histone proteins and forms a condensed structure called chromatin. The order is of assembly is -

a) HISTONE CORE

b) HISTONE PROTEINS

c) NUCLEOSOMES AS BEAD ON A STRING

d) 30NM CHROMATIN FIBRE

e) PROTEIN FILLAMENT SCAFFOLD

f) RADIAL LOOP DOMAIN

2)

In Eukaryotic DNA replication, DNA polymerases alpha, delta and epsilon play a very crucial role.

DNA polymerase lays down the primers on which DNA synthesis is carried out. DNA polymerase delta and epsilon then take over the function of replication. In addition to DNA polymerases several other DNA binding proteins, clamp loader protein, PCNA are required for this process.

DNA polymerases delta is responsible for the lagging strand synthesis thus it will be most effected by the mutation because it will miss out bases at start of each okazaki fragment.

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