On Tuesday morning. Caitlyn went immediately to see her pediatrician. The lesion

Question

On Tuesday morning. Caitlyn went immediately to see her pediatrician. The lesion was the size of an egg and quite sore Caitlyn also presented with a temperature of 38.4°C (101.2 F). Motrin and compresses were advised as comfort measures. The Keflex was continued and the lesion cultured for laboratory analysis. Again, she was told to return if she didn't notice improvement 5. How would you collect a specimen from Caitlyn's lesion? Name several types of transport media commonly used. Why is it so important to appropriately transport a specimen to the microbiology laboratory? 6. What media will likely be inoculated when this sample arrives in the laboratory? State your reason(s) for choosing the media you've indicated. 7. In addition to media inoculation, what other procedure will be performed immediately using the specimen? The preliminary Gram stain of the specimen showed many Gram-positive cocci in clusters. After 24 hours, the TSA with 5% sheep blood plate demonstrated pure growth of small, round, smooth, white, gamma-hemolytic colonies. The same colony morphology was observed on the PEA (or CNA) plate with zero growth on the EMB (or MacConkey) plate. Colonies were also observed on the MSA plate, which was completely pink in color 8. What is meant by the term "pure growth"? What does it say regarding the quality of your specimen collection? 9. Based upon these laboratory results, what microbe do you predict is causing Caitlyn's infection? Explain. What two chemical tests would you perform next to verify your answer? Colonies from the TSA plate were suspended in sterile saline and introduced into the Vitek II analyzer. It confirmed Staphylococcus epidermidis was the pathogen involved and indicated Keflex sensitivity

Explanation / Answer

Ans7:

The specimen will be used for identification in addition to media inoculation.

Identification will help in characterization of the bacteria involved.

The media is inoculated so that a culture of bacteria is made for further studies.

Ans 8:

Pure growth means that the culture derived from inoculation of media contains population derived from a single specific bacterial strain (or species).

[Or, pure growth refers to a culture of bacteria containing a single specific strain or species.]

If the culture has pure growth, then specimen collection is free of contamination and of good quality. However, if pure growth is not achieved, then specimen collection is of poor quality and involves errors/contamination.

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