(25 points) DNA construct A is an expression construct that can express cytosoli

Question

(25 points) DNA construct A is an expression construct that can express cytosolic protein X of a full length of 200 amino acids when delivered into cultured mouse cells. a. If construct A is engineered into construct B that can encode protein X with an "mitochondrial 4. intermembrane-space-targeting sequence" (gray box in the graph below) inserted into the middle of the protein, where would the new protein X go during its protein-sorting journey? Why? If construct B is further engineered into construct C that can encode protein X with an additional ER signal peptide (black box in the graph below) at the N-terminus, where would the new protein Xgo during its protein-sorting journey? Why? b. protein encoded by construct B (N-terminus)(C-terminus) protein encoded by construct C

Explanation / Answer

if construct A is engineered into construct B, then the expression of construct B is inhibited and the expression of construct A is continued in the protein-sorting journey.

if construct B that contains construct A is inserted into the construct C, then the expression of construct C is also inhibited along with the inhibition of expression of construct B and only the expression of construct A is allowed.

This happens because of the interruption of the continuation of the gene sequence of both the constructs of B and C.

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