7. Pro karyotes and eukaryotes carry out transcription using very similar machin

Question

7. Pro karyotes and eukaryotes carry out transcription using very similar machinery, but there are also distinct differences 7a. Circle the components below that are present in eukan RNA Polymerase Promoter Enhancer Splicing 5' Cap 3 pts] Poly (A) Tail Mediator TATA box Terminator Sigma Factor 7b. When a region of DNA that contains the genetic information for a protein is isolated from a bacterial cell and inserted into a eukaryotic cell in a pro resulting cell usually produces direction (eukaryotic DNA into a bacterial cell), the correct protein is often not produced even though transcription is carried out. The absence of which of the component(s) in 7a in bacteria may explain this observation? [4 pts per position between a promoter and a terminator, the the correct protein. But when the experiment is done in the reverse

Explanation / Answer

prokaroytic transcription -takes place in nucleus ,have one type of RNA polymerase ,trancsciption initiation machinery is simple as dna not linked with histone proteins ,contains sigma factor ,tata box absne in promoter region, RNA capping absent.

eukaryotic - occurs in cytoplasm , have 3 types RNA polymerase one , two and three. , initiation machinery complex as genetic material linked with protein ,sigma factor absent , possess tata box, RNA capping occurs at 5' position mRNA.

NOT FOUND IN BACTERIA BUT IN EUKARYOTES -TATA box,splicing

abscenc eof sigma factor is the reason.

it is needed for initiation of transcription that helps in specific binding of RNA polymerase to gene promoters.

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