The PV92 locus sequence with the Alu insert in bold is: AACTGGGAAAATTTGAAGAGAAAG

Question

The PV92 locus sequence with the Alu insert in bold is:

AACTGGGAAAATTTGAAGAGAAAGTCACACAGATACATTTCAGTAAGGTTGTCTCTGTTACTTGAGGCTTACAAGAAGGAAAGAAGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCCGGCTAAAACGCTGAAACCTCGTCTCTACTAAAAATACAAAAAATTAGCCGGGCGTAGTGGCGGGCGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCCTGCCACTGCACTCCAGCGTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAGAAAGAATTCCCTCTCTAAACACACTCTAACACACAGGAGTTGAGAACTCA

construct a pair of primer sequences (approximately 25bp) that would amplify any Alu sequence inserted in the genome. Assume that all Alu sequences have the same sequence. Remember when designing primers, one of the pair is complimentary to one strand of DNA, and the second is complimentary to the other strand of DNA. Also, the primers are designed from the 5’ to the 3’ direction.

Explanation / Answer

Forward primer: 5' GATCGAGACCATCCCGGCTAAAACG 3'

Reverse primer: 5' CAGGATCTCGGCTCACTGCAAGCT 3'

Amplicon size 165bp (approx.)

Alu sequences are repetitive DNA sequences approx 300bp in length and they contribute almost 10% of human genome. They are the most abundant transposable elements.

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