3) In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary trac
ID: 151034 • Letter: 3
Question
3) In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary tract of 10 percent of 1300 patients who underwent gastrointestinal endoscopic procedures. After each use, endoscopes were washed with an automatic reprocessor that flushed detergent and glutaraldehyde through the endoscopes, followed by a tap water rinse. P. aeruginosa serotype 10 was not isolated from the detergent, glutaraldehyde, or tap water. What was the source of the infections? A) bacterial cell walls in the water B) a biofilm in the reprocessor C) contaminated disinfectant D) fecal contamination of the bile ducts E) None of the answers is correct 4) A biochemical test, in the microbiology world, is used to determine A) staining characteristics B) amino acid sequences of enzymes. C) nucleic acid-base composition of DNA sequences. D) capability of a microbe to perform a specific enzymatic activity. E) All of the answers are correct. 5) Which one of the following provides taxonomic information that includes the information obtained in each of the other options listed? A) nucleic acid hybridization B) nucleic acid-base composition C) amino acid sequencing D) biochemical tests E) cladogramExplanation / Answer
Answer 3: Option B is correct. As biofilms are microbial masses attahed to the surfaces of equipments that are come in continous contact of water. Here machine (automated reprocessor) is self contaminated. So it is the source of infection.
Option A is incorrect as bacterial cell wall cannot cause infection. option C is also incorrect as it is already mentioned in the problem P. aeruginosa serotype 10 was not isolated from detergent, glutaraldehyde and tap water. There is no sense of option D and E in this problem.
ANSWER 4: Option D is correct.
As biochemical test is used to identify the microbial species based on their different biochemical activities. Biochemical activities identified by the study of specific enzymatic activity.
And this explanation is well matched with option D.
ANSWER 5: Option E is correct.
Explanation: Cladogram is a diagram used for the study of the relation among organism and provides taxanomic information.
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