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1. What will be produced from a prokaryotic operon? a. Multiple proteins made fr

ID: 151217 • Letter: 1

Question

1. What will be produced from a prokaryotic operon?

a. Multiple proteins made from a single RNA transcript

b. A separate mRNA for each gene in the operon

c. A spliced RNA transcript

d. A single, large protein that contains multiple domains

2. What do microRNAs and siRNAs have in common?

a. They both act as part of an RNA-induced silencing complex

b. They both are used to inhibit the transcription of genes

c. They both increase how tightly nucleosomes are packed together

d. They both form covalent bonds to mRNA molecules

e. All of the above

3. In the lab, you engineer a new DNA molecule where you put the promoter of the lac operon in front of the trp operon (which encodes enzymes to synthesize the amino acid tryptophan). The normal trp promoter is not present in this DNA molecule. What will occur when you put this new DNA into a bacterial cell?

a. High levels of tryptophan will increase the production of enzymes that digest lactose

b. Low levels of tryptophan will signal to the cell to make more lactose-digesting enzymes

c. If lactose is present and glucose is available, enzymes needed to synthesize tryptophan will be made from this operon.

d. If lactose is present and glucose is unavailable, enzymes needed to synthesize tryptophan will be produced.

a. Multiple proteins made from a single RNA transcript

b. A separate mRNA for each gene in the operon

c. A spliced RNA transcript

d. A single, large protein that contains multiple domains

2. What do microRNAs and siRNAs have in common?

a. They both act as part of an RNA-induced silencing complex

b. They both are used to inhibit the transcription of genes

c. They both increase how tightly nucleosomes are packed together

d. They both form covalent bonds to mRNA molecules

e. All of the above

3. In the lab, you engineer a new DNA molecule where you put the promoter of the lac operon in front of the trp operon (which encodes enzymes to synthesize the amino acid tryptophan). The normal trp promoter is not present in this DNA molecule. What will occur when you put this new DNA into a bacterial cell?

a. High levels of tryptophan will increase the production of enzymes that digest lactose

b. Low levels of tryptophan will signal to the cell to make more lactose-digesting enzymes

c. If lactose is present and glucose is available, enzymes needed to synthesize tryptophan will be made from this operon.

d. If lactose is present and glucose is unavailable, enzymes needed to synthesize tryptophan will be produced.

Explanation / Answer

I believe the correct answer to be:

1) a. Multiple proteins made from a single RNA transcript which is also observed in lac operon.

2) a. They both act as part of an RNA-induced silencing complex

3) d. If lactose is present and glucose is unavailable, enzymes needed to synthesize tryptophan will be produced. because presence of lactose is needed to inhibit lac repressor while absence of glucose is required for activation of CAP protein.

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