16. In phase contrast microscopy, what is the main purpose of using annular ring

Question

16. In phase contrast microscopy, what is the main purpose of using annular ring and phase ring? a. Annular Ring: to reduce the wavelength of the light Phase Ring: to magnify the image Phase Ring: to focus and send image to the image plate Phase Ring: To collect deflected lights only Phase Ring to cause phase shift by having two different pathways with different thicknesses Phase Ring: to cause the phase shift by passing the light through one pathway b. Annular Ring: to reduce the brightness of the light c. Annular Ring: to focus the light on the specimen d. Annular Ring: to distinguish perturbed and unperturbed light passing through the specimen/ e. Annular Ring: to increase the wavelength of the light

Explanation / Answer

The main purpose of using annular ring and phase ring is

c) Annular ring: To focus the light on the specimen

Phase ring: To collect deflected light only

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