0.91 Only our group\'s % error for P200 and % error for P1000 In general, microp
ID: 151501 • Letter: 0
Question
0.91
Only our group's % error for P200 and % error for P1000
In general, micropipettes are designed to function with accuracies less than 3% of the intended value. Determine the % error for each of your aliquots with the P200 and the P1000. Percent error is calculated as follow:
(Actual micro liter - Intended micro liter) / intended micro liter * 100
a) What may have caused you to hav a percent error?
Please Help Me! :)
Class Average Percent + Standard Deviation Error Average % error SD of error P200 2.06 0.78 P1000 1.620.91
Explanation / Answer
Accuracy is described as: the variation of the measured value from the actual value or close to the standard deviation or accepted value.
Causes of percent error while using micropipette:
The micropipette should be ideally held vertically. Pipette held within 30 ° angle off the vertical plane, may cause about 0.7 % error.
The micropipette tips should be pre-wetted or rinsed in the sample before actual pipetting, to remove any air.
If the plunger is released abruptly, or in a bumpy manner, some liquid would remain accumulated within the pipette and released later with other sample. This causes variability in reading.
The micropipette tips should not be immersed in sample beyond 1-3 mm. Otherwise the liquids adhere more to the tips causing more volume.
Dead air may get incorporated due to thermal conductance.
Over-dialing or under dialing of micropipette settings and erroneous readings.
Temperature, humidity or barometric pressure may cause variation in liquid reading. Like temperature may cause expansion or contraction. Pressure may result in lowering or increased liquid level. Humidity may cause moisture variation.
The sample should be dispensed by touching the tip of the micropipette to the side of the receiving tube and not into thin air, as surface tension would help in complete dispense.
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