Sprint? 4:14 AM bimu.docx Biology 1406 Lab report 1: Identification of Biologica

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Sprint? 4:14 AM bimu.docx Biology 1406 Lab report 1: Identification of Biological Back Molecules Lab instructor Goal: This experiment was mainly performed to identify the biological molecules present in unknown solutions (mixtures). Known as well as unknown solutions were tested (specific test) to find the presence or absence of four biomolecules (starch, reducing sugar, protein and lipid). Hypothesis: The solutions will show change in their color and appearance after reaction with specific reagents. Their color and appearance will notify about the presence or absence of biomolecules. Materials Required: 16 clean and dry test tubes Test tube rack 450 ml beaker Test tube holder (clamp) Hot plate Disposable pipet Boiling chips Wax pencil 4 Known solutions:- a. Soluble liquid starch b. 1% glucose solution c. Corn oil d. Albumin (egg white) Deionized water (dH20) Iodine reagent in dropper bottle Biuret reagent in dropper bottle Benedict's reagent in dropper bottle Sudan IV reagent in dropper solution 2 unknown solutions Procedure/ Methods: Part 1 First of all, about half amount of water was taken on a 450 ml beaker and heated on a hot plate using few boiling chips. Then the temperature was lowered after the water boiled completely and other procedures were started. 16 clean and dry test tubes were then taken on a test tube rack. After this, 8 test tubes were taken and labelled as A1, B, CI, DI, A2, B2, C2 and D2 with a wax pencil. A, B, Cl and D test tubes were filled with required amount (about 2ml) of corn starch solution, reducing sugar solution, albumin (white egg) and corn oil respectively. While A2, B2, C2 and D2 were filled with same amount of

Explanation / Answer

Lab report 1 biology1406 : biological molecules identification.

GOAL: In order to identify the biological molecules in mixture solution which is unknown this experiment was made. Identified and unidentified solutions are the tester to find out basic biomolecules that are carbohydrates, protein, and lipids.

Hypothesis: After the reduction process with the particular reagents solution show change in the colour.

We can identify specific biomolecules by the change in colour of the solution.

Material needed:

• Dry and unaffected test tube: 16

• Rack of the test tube

• Beaker:450 ml

• Hotplate

• Pipet disposable

• Chips(boiling)

• Pencil(wax)

• Unidentified solutions: 4

• Liquid soluble starch

• 1% glucose solution

• Egg white protein

• Oil of corn

• dH2O

• Iodine regent

• Biuret reagent

• Benedict reagent

• Unidentified solution

• Sudan 4 reagent

Procedure:

First part:

At first, half part of water is inserted in the beaker of 450ml and boiled on the hot plate by using chips(boiling).After boiling of water decrease the temperature and start another process.

On the test tube rack, 16 test tube was taken.

With the wax pencil each 8 test tube were labelled :A1,B1,C1,DE and A2 ,B2,C2,D2.

First 4 test tube was filled with starch, glucose, lipid, and protein(egg albumin and others were filled with deionized water.

Starch: iodine is inserted into two test tube if starch is present colour changed from yellow to purple.

Benedict test:5 drops reagent is inserted into two test tube if glucose is present colour changed from blue to orange.

Bitrate test: 5 drops reagent is inserted into two test tube if the protein is present colour changed from light blue to violet.

Sudan 4 test: 5 drops reagent is inserted into two test tube if the lipid is present, pink precipitation appear.

Observation: test tube a1,b1,c1, and d1 shows the change in colour and other groups do not show any change in colour.

Procedure second:

Other parts of the test tube were takin and With the wax pencil, each 8 test tube were labelled:A1, B1, C1, D1 and A2, B2, C2, D2.

First 4 test tube was filled with starch, glucose, lipid, and protein(egg albumin and other were filled with deionized water.

Repeat above test on these.

Observation: test tube a1,b1,c1, and d1 shows the change in colour and other groups do not show any change in colour.

Conclusion: We can detect any solution by these methods because the change in colour shows that biomolecules are present in solutions.

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