The following figure shows an experiment looking at wildtype (Usp9x) and knockou

Question

The following figure shows an experiment looking at wildtype (Usp9x) and knockout (Usp9x) mouse hippocampal neurons. UsD9x Usp9xN Control Control USP9X 1. What does the +/Y and -/Y mean when describing the Usp9x genotype? (1 mark) 2. Figure 1 shows neurons that have been fluorescently labeled. Briefly describe an experimental approach that could be used to achieve this labelling. (2 Marks) 3. What observations can be made about the neuron phenotype in the presence or absence of the USP9X protein? (2 Marks)

Explanation / Answer

1. +/Y represent that in this case Usp9x gene is present whereas in -/Y represent Usp9x is missing in the mutant one.

2. Experimental approach is direct fluorescent labelling where dye is going bind with proteins express by Usp9x gene and the expression of this gene or it's protein is measured by fluroscent emission.

3. Two observation could be made

a. Reduce length of axon

b. Arborization of neuon

c. Low neuronal migration

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