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. An enzyme, alphabetase, catalyzes this reaction: C D. Researchers find that th

ID: 151931 • Letter: #

Question

. An enzyme, alphabetase, catalyzes this reaction: C D. Researchers find that the the Km for the substrate C is 7uM, and the Kcat is 800 s-1.

Suppose alphabetase actually catalyzed the conversion of C (below, on the left) to D (below, on the right). How would you classify this enzyme and why? What coenzyme might be required for this?

. Suppose alphabetase actually catalyzed the conversion of C (below, on the left) to D (below, on the right). How would you classify this enzyme and why? What coenzyme might be required for this?

OH OH OH OH OH OPO3 CH2-C-CH2 CH C CH2 Glycerol Glycerol 3-phosphate

Explanation / Answer

1. Given reaction,
Glycerol -----> Glycerol-3-phosphate
It is a phosphorylation reaction.
It is catalyzed by Glycerokinase. It is a class II enzyme (Transferase).

1. Given reaction,
Glycerol -----> Dihydroxyacetone
It is a dehydrogenation reaction.
It is catalyzed by Glycerol dehydrogenase.
It requires NAD+ as the cofactor.