3. Bioseparations (5 points) A. Many important proteins are isolated from human
ID: 15199 • Letter: 3
Question
3. Bioseparations (5 points)A. Many important proteins are isolated from human serum. The first step is usually salt fractionation (sometimes called fractionational precipitation). Explain in 2-3 lines what this is. (3 points)
B The next step is isoelectric point precipitation. Describe what this is in one sentence (1 point)
B. Immunoaffinity chromatography is then use to further separate serum proteins that are present in low concentrations such as the anti-clotting protein Factor X. Explain in one sentence how this technique works (1 point).
Explanation / Answer
I was a little confused by your question, but I tried to get you some good into.... Hope this help!! A) Fractionation is a separation process in which a certain quantity of a mixture (solid, liquid, solute, suspension or isotope) is divided up in a number of smaller quantities (fractions) in which the composition changes according to a gradient. Fractions are collected based on differences in a specific property of the individual components. A common trait in fractionation is the need to find an optimum between the amount of fractions collected and the desired purity in each fraction. Fractionation makes it possible to isolate more than two components in a mixture in a single run. This property sets it apart from other separation techniques. B) Precipitation of materials at the isoelectric point (the pH at which the net charge on a molecule in solution is zero); proteins coagulate best at this point. C) Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique.
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