9. Does the microorganism form 10. What types of dentification procedures are us
ID: 152070 • Letter: 9
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9. Does the microorganism form 10. What types of dentification procedures are used for visualization of this m spores? If so, describe the spores and their arrangement microorganism? so, describe a. F or many microorganisms, this will include staining procedures. For others, it may be strict microscopic observation, either with a light or electron microscope. You should discuss why these particular identification procedures microorganism. work with this 11. Are there any growth limitations to this microorganism? If so, what are they? Be sure to include what special nutrients the organism needs and what its preferred growth conditions are (we will discuss how environmental conditions such as pH, temperature, etc. affect growth). a. 12. Can you culture the microorganism in the lab? If so, how? 13. What is a typical generation time for the microorganisms? 14. How does it reproduce? 15. What is the metabolism of the microorganism like? a. Is the microorganism an autotroph or heterotroph? 16. What is the genetic material of this species like? How many chromosomes does it have, if any? Is/Are the chromosome(s) circular or linear? Does it rely upon plasmids? If so, what benefits do they provide? a. 17. Briefly discuss the classification of your microbe. This will differ depending upon whether you have a bacterium, a virus, or a eukaryote. What characteristics are generally used to classify your microbe as a member of a particular family/class/order/genus/species? 18. What relationship does your microbial species have with humans? Is it part of the normal flora? Is it always pathogenic or on in particular circumstances? 19. Does your microorganism have any positive attributes to either humans or the environment? 20. What disease(s) is/are cause by the microorganism? 21. What are the symptoms of this disease? 22. Are there any possible complications from infection with this microorganism? 23. How is the microorganism transmitted humans? 24. What are the risk factors? In other word, what makes someone susceptible to infect 25. What is the length of time from infection to onset? 26. What is the total length of time of infection? 27. What is the average number of cases globally? 28. What is the prevalence of cases in the US? 29. Where are cases of this infection primarily reported? 30. When are cases primarily reported?Explanation / Answer
9. Shigella dysenteriae is a non-spore forming bacillus bacteria that survives as facultative anaerobes. They are members of Enterobacteriaceae.
10. a ) There are various identification steps such as they produce ink colonies on XLD medium and produce colourless colonies on DCA. It is also catalase positive and ONPG positive. Isolates from primary isolates are cultures by their colonial appearance, biochemical test and serological tests.
11. Shigella mainly grow in medium containing methionine, nicotinic acid and tryptophan. The combination of methionine and tryptophan appears to be an obligatory requirement for Shigella dysentriae serotype 1 while a combination of nicotinic acid and tryptophan appears to be obligatory for serotype 2. The growth and survival of this species are limited by various factors such as temperature, pH, salt etc. The optimum temperature range for the growth is between 6-8 to 45-47 degree Celsius. Rapid inactivation occurs at 65 degrees.
pH range is between 5-9. Shigella species grow better at lower pH than at low temperature. Shigella species are also able to grow at the various surface. S.dysentriae is also shown to survive on the surface including plastic, glass, aluminium etc.
12. They can be grown in the laboratory provided that the following conditions be met:
13. Thir typical generation time is 40 minutes. Incubation time is average 3 days.
14. Reproduction is mainly the means of conjugation . This facilitates the transfer of DNA from the donor to the recipient cell.
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