1 During a multi-step purification protocol, what would you expect to happen to

Question

1 During a multi-step purification protocol, what would you expect to happen to the specific activity at each step?

Select one:

a. Stay the same

b. Decrease

c. Increase

2. In separation by size-exclusion chromatography, which species of protein would be the fastest to elute?

Select one:

a. Largest

b. More positively charged

c. More negatively charged

d. Smallest

e. Most spherical shape

3.

Native PAGE is similar to SDS-PAGE analysis, except is performed in the absence of SDS. Johnny has a 60 kDa trimer protein and a 40 kDa dimer protein. How would these two proteins run on the gel?

Select one:

a. The same distance

b. The second protein would run further

c. The first protein would run further

4.

What type of column separates based on charge?

Select one:

a. Ion exchange

b. IMAC

c. Size exclusion

Explanation / Answer

1.The specific activity will increase as the protein is getting purified with each proceeding step and our protein of interest is getting abundant.

So (c) is correct option.

2.In separation by size-exclusion chromatography, the largest molecules will elute first as they do not get trapped into the pores and travel through interstitial spaces between the beads.

So (a) is correct option.

3.In native page as the protein is visulaized as a whole so the protein with small size will run further as compared to protein with bigger size.Therfore, 40 kDa dimer protein will move fast.

The correct option is (b).

4.The chromatography based on charge is Ion exchange chromatography.

So (a) is correct option.

Hope it helps, Good luck !!!

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