Jane and Hemi had a solution containing four proteins and analysed it by SDS-PAG
ID: 152154 • Letter: J
Question
Jane and Hemi had a solution containing four proteins and analysed it by SDS-PAGE. The four proteins A, B, C and D had subunit molecular weights of 10 kDa, 20 kDa, 30 kDa and 15 kDa respectively, with the quaternary structures of the proteins being monomeric, tetrameric, dimer and tetrameric, respectively. Which would travel the furthest on the gel (in order of most distance to least distance)? Second least distanceose ' Most distance Second most distance Choose.. Least distance Choose...v Choose Choose.Explanation / Answer
Second least distance: 15kDa
Most distance: 30KDa
Second most distance: 20kDa
Least distance: 10kDa
Separation of proteins based on their molecular weight. Different proteins in their native state with the same molecular weight would migrate at different speeds in an electrical field depending on their charge and 3D shape.When the proteins are running in the gel, they get separated based on their molecular weight (mw), where high mw protiens move more slowly through the porous acrylamide gel than lower molecular weight proteins. The size of the pores in the gel can be altered depending on the size of the proteins you want to separate by changing the acrylamide concentration.
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