1. Immediately after the poster presentations, Aaron and Kyle decide to take an
ID: 15922 • Letter: 1
Question
1. Immediately after the poster presentations, Aaron and Kyle decide to take an impromptu trip to Yellowstone. It is a long drive and as they make their way up north and, Aaron (not unexpectedly) begins to tease Kyle, “Kyle, you remind me the most of a microorganism in Volume 1 of Bergey’s Manual of Systematic Bacteriology. Thismicroorganism can take a lot of heat, in fact its optimal growth temperature is 181.4ºF. However, it is also quite a nuisance as it can cause corrosion of iron and steel in gas processing systems by forming FeS.” To which microorganism is Aaron referring
a. Sulfolobus solfataricus
b. Clostridium deficil
c. Desulfovibrio desulfuricans
d. Pyrococcus furiosus
e. Archaeoglobus fulgidus
2. Kyle, ignoring Aaron, returns his attention to his Microbiology textbook where he is
reading about the Thermoproteus tenax that he hopes to observe in Norris Geyser Basin.Kyle reads all of the following about T. tenax EXCEPT?
a. T. tenax is a thermoacidiphilic member of the phylum Crenarchaeota.
b. T. tenax has phospholipids that differ from those of thermophilic bacteria. Namely
the linkages that connect the hydrocarbon side chains to the glycerol backbone are
different (ether rather than ester linkages).
c. The peptidoglycan of T. tenax contains unusual D-amino acids that cross-link the
N-acetylmuramic acid residues of the glycan chains.
d. The genes of T. tenax are under control of promoters that are similar to eukaryotic
promoters.
3. Kyle and Aaron arrive in West Yellowstone, pack their backpacks (Kyle carefully places his Microbiology text into the top of his pack), and clip into their skis. As they ski towards Madison Junction, Aaron notices the thick mat of Cyanobacteria along
Yellowstone River. The two stop and Kyle pulls out a tiny microscope that Aaron did not even know he had. After observing the bacteria in the matt, Kyle states that it looks like Nostoc and that he can see the heterocysts. Aaron immediately realizes that these heterocysts
a. lack the ability to oxidize water.
b. contain the enzyme nitrogenase.
c. cannot perform noncyclic photophosphorylation.
d. provide ammonia to other cells of the trichome.
e. all of the above
4. Aaron and Kyle hit the skis again and by the time the sun is setting, they have made their way to Norris Geyser Basin. After setting up their tents, Kyle pulls out some “light” bedtime reading: Since its original discovery in Congress Pool, a thermal feature at Yellowstone’s Norris Geyser Basin with an average temperature of 80oC and average pH of 3.0 (Brock et al. 1972), species of the genus ______________ have been isolated from various solfataric fields [in] Pisciarelli, Italy (Zillig et al. 1980);
…Sogasel, Iceland (Zillig et al. 1994); and … from Japan’s Beppu Hot Springs (Suzuki et al. 2002)._____________ species are generally aerobic and [although] heterotrophic growth has been reported, autotrophic—oxidation of S2O32-, S4O62-, So and S2- to sulphuric acid,… has been [primarily] described (Shivvers and Brock 1973; Schönheit and Schäfer 1995). (Brouns et. al., Geothermal Biology and Geochemistry in Yellowstone National Park) Kyle reads this passage out loud and tells Aaron to fill in the blank with the correct genus name
a. Methanococcus
b. Thermoproteus
c. Picrophilus
d. Sulfolobus
e. Halobacterium
5. The next morning Aaron and Kyle wake up bright and early to the smell of H2S gas and the sound of skis on the snow. They pop their heads out of their tents and see Nikki andJeslyn ski up. Aaron can just overhear Nikki and Jeslyn discussing certain
microorganisms. Of all of the statements that Aaron overhears, which is the only
INCORRECT statement?
a. “Although found in the environment, it would be easiest to isolate Paleococcus
from the feces of a ruminant.”
b. “Thermoplasma could be isolated from a refuse pile at a coal mine.”
c. “Halobacterium salinarium could be isolated from the Great Salt Lake or the
Dead Sea.”
d. “Because Picrophilus can tolerate more acidic environments, it would be more
likely to be found in certain solfateras where the pH drops below 1.”
6. Aaron and Kyle decide to join Nikki and Jeslyn as they all walk around the boardwalk of Norris Geyser basin. On their walkabout
a. they observe red zones created by the pigments of Halobacterium salinarium.
b. they smell methane gas produced by the resident population of Desulfovibrio.
c. they recognize yellow zones in which they know a resident population of
thermoacidophilic members of the phylum Crenarchaeota live.
d. All of the above
e. A and B
7. The beauty of the microbial zones is, at this point, simply too much for Aaron to take and he stops on the boardwalk and declares that he is going to get a PhD in microbiology and specialize in archaeal metabolism. Nikki thinks this sounds like a pretty good plan and reminds Aaron that with such a credential he could work for/in ________________.
a. academia
b. medical research
c. industry2
d. NASA (http://exobiology.nasa.gov/)
e. All of the above
8.Unlike fungi, plants and animals, protists
a. lack complex tissue organization.
b. are prokaryotes
c. contain peptidoglycan in their cell walls
d. are always photoheterotrophic.
9. Giardia intestinalis
a. utilizes aerobic respiration to generate energy.
b. is a trypanosome.
c. is in the supergroup Excavata.
d. is found only in marine environments.
10. Hannah P. decides to spend her day out riding fences and checking on the cattle tanks.After visiting two of the tanks, Hannah becomes concerned as she sees a red surface bloom on both. She remembers from microbiology that this “bloom” is
a. caused by Euglena.
b. caused by Trypanosoma gambiense.
c. caused by a protist that is motile via pseudopodia.
d. the primary cause of amoebic dysentery in humans.
Explanation / Answer
1. Immediately after the poster presentations, Aaron and Kyle decide to take an impromptu trip to Yellowstone. It is a long drive and as they make their way up north and, Aaron (not unexpectedly) begins to tease Kyle, “Kyle, you remind me the most of a microorganism in Volume 1 of Bergey’s Manual of Systematic Bacteriology. This
microorganism can take a lot of heat, in fact its optimal growth temperature is 181.4ºF. However, it is also quite a nuisance as it can cause corrosion of iron and steel in gas processing systems by forming FeS.” To which microorganism is Aaron referring
e. Archaeoglobus fulgidus
2. Kyle, ignoring Aaron, returns his attention to his Microbiology textbook where he is
reading about the Thermoproteus tenax that he hopes to observe in Norris Geyser Basin.Kyle reads all of the following about T. tenax EXCEPT?
a. T. tenax is a thermoacidiphilic member of the phylum Crenarchaeota.
b. T. tenax has phospholipids that differ from those of thermophilic bacteria. Namely
the linkages that connect the hydrocarbon side chains to the glycerol backbone are
different (ether rather than ester linkages).
c. The peptidoglycan of T. tenax contains unusual D-amino acids that cross-link the
N-acetylmuramic acid residues of the glycan chains.
d. The genes of T. tenax are under control of promoters that are similar to eukaryotic
promoters.
3. Kyle and Aaron arrive in West Yellowstone, pack their backpacks (Kyle carefully places his Microbiology text into the top of his pack), and clip into their skis. As they ski towards Madison Junction, Aaron notices the thick mat of Cyanobacteria along
Yellowstone River. The two stop and Kyle pulls out a tiny microscope that Aaron did not even know he had. After observing the bacteria in the matt, Kyle states that it looks like Nostoc and that he can see the heterocysts. Aaron immediately realizes that these heterocysts
a. lack the ability to oxidize water.
b. contain the enzyme nitrogenase.
c. cannot perform noncyclic photophosphorylation.
d. provide ammonia to other cells of the trichome.
e. all of the above
4. Aaron and Kyle hit the skis again and by the time the sun is setting, they have made their way to Norris Geyser Basin. After setting up their tents, Kyle pulls out some “light” bedtime reading: Since its original discovery in Congress Pool, a thermal feature at Yellowstone’s Norris Geyser Basin with an average temperature of 80oC and average pH of 3.0 (Brock et al. 1972), species of the genus ______________ have been isolated from various solfataric fields [in] Pisciarelli, Italy (Zillig et al. 1980);
…Sogasel, Iceland (Zillig et al. 1994); and … from Japan’s Beppu Hot Springs (Suzuki et al. 2002)._____________ species are generally aerobic and [although] heterotrophic growth has been reported, autotrophic—oxidation of S2O32-, S4O62-, So and S2- to sulphuric acid,… has been [primarily] described (Shivvers and Brock 1973; Schönheit and Schäfer 1995). (Brouns et. al., Geothermal Biology and Geochemistry in Yellowstone National Park) Kyle reads this passage out loud and tells Aaron to fill in the blank with the correct genus name
a. Methanococcus
b. Thermoproteus
c. Picrophilus
d. Sulfolobus
e. Halobacterium
5. The next morning Aaron and Kyle wake up bright and early to the smell of H2S gas and the sound of skis on the snow. They pop their heads out of their tents and see Nikki andJeslyn ski up. Aaron can just overhear Nikki and Jeslyn discussing certain
microorganisms. Of all of the statements that Aaron overhears, which is the only
INCORRECT statement?
a. “Although found in the environment, it would be easiest to isolate Paleococcus
from the feces of a ruminant.”
b. “Thermoplasma could be isolated from a refuse pile at a coal mine.”
c. “Halobacterium salinarium could be isolated from the Great Salt Lake or the
Dead Sea.”
d. “Because Picrophilus can tolerate more acidic environments, it would be more
likely to be found in certain solfateras where the pH drops below 1.”
6. Aaron and Kyle decide to join Nikki and Jeslyn as they all walk around the boardwalk of Norris Geyser basin. On their walkabout
a. they observe red zones created by the pigments of Halobacterium salinarium.
b. they smell methane gas produced by the resident population of Desulfovibrio.
c. they recognize yellow zones in which they know a resident population of
thermoacidophilic members of the phylum Crenarchaeota live.
d. All of the above
e. A and B
7. The beauty of the microbial zones is, at this point, simply too much for Aaron to take and he stops on the boardwalk and declares that he is going to get a PhD in microbiology and specialize in archaeal metabolism. Nikki thinks this sounds like a pretty good plan and reminds Aaron that with such a credential he could work for/in ________________.
a. academia
b. medical research
c. industry2
d. NASA (http://exobiology.nasa.gov/)
e. All of the above
8.Unlike fungi, plants and animals, protists
a. lack complex tissue organization.
b. are prokaryotes
c. contain peptidoglycan in their cell walls
d. are always photoheterotrophic.
9. Giardia intestinalis
a. utilizes aerobic respiration to generate energy.
b. is a trypanosome.
c. is in the supergroup Excavata.
d. is found only in marine environments.
10. Hannah P. decides to spend her day out riding fences and checking on the cattle tanks.After visiting two of the tanks, Hannah becomes concerned as she sees a red surface bloom on both. She remembers from microbiology that this “bloom” is
a. caused by Euglena.
b. caused by Trypanosoma gambiense.
c. caused by a protist that is motile via pseudopodia.
d. the primary cause of amoebic dysentery in humans.
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