I did a lab on identification of unknown digestive juices, I had 4 unknowns, her

Question

I did a lab on identification of unknown digestive juices, I had 4 unknowns, here is the procedure and my results, I was not sure what the juices were from my results. I am confused because I thought there 3 digestive juices but I have four unknowns, I am also unsure if enzymes and digestive juices are the same, I dont think so. Help please, Also I didnt include a control how can I innclude inn my lab report tho?

1. Four unknown digestive juices were pre-incubated for 5 minutes. 2. After the incubation period, 4 sets of test tubes were placed in a test tube holder and labelled A, B, C, and D. Next, a 3ml-to-3ml-to-3ml ratio of digestive juice to buffer to substrate content was added to each tube via pipette. This was repeated four times. In the test for lipids a 3:3:1 ratio was done and bile was added 3. Each tube was mixed thoroughly via vortex. 4. Next, each set of test tubes were then incubated. Starch was incubated for 15 minutes while cream was incubated for 20-25 minutes and albumin for 45 minutes. The incubation chamber was set up prior to the lab at 37 ºC. 5. After the incubation period, all tubes were tested for the presence of starch, protein digestion and lipid digestion. For starch, the IKI solution was utilized. 1 drop of IKI to 3 drops of starch solution in a spot plate. A color change from amber-yellow to blue-black indicates a positive test result. For protein, the visual clarity test was performed—since albumin is cloudy, any clearing would indicate some degree of digestion. This was done at two different pH. pH 2 for stomach environment and pH 8 exhibiting small intestine environment at ph 8. A phenol red test was performed to test for lipid digestion. 20 drops of phenol red solution were added to 3 ml of cream. A color change from pink (fatty acids absent) to yellow (fatty acids present) indicates extent of lipid digestion

Results STARCH TEST |LIPID TEST PROTEIN TEST-PROTEIN TEST UKNOWN DIGESTIVE WITH CREAM: [WITH-am JUICES ALBUM IN-PH2-ALBUM LARnv CLARITY D #MISU LARITY +DIGESTED, Iiicillo

Explanation / Answer

According to the given details and results obtained, the unknown digestive juices are identified as below

A - Lipase

B - Pepsin & Trypsin

C - Amylase

D- Pepsin & Trypsin

Lipase acts on the cream, hence it produced a positive test with the lipid and starch test.

Amylase digests the starches present in the solution, and hence a positive for starch test.

Pepsin & Tyrpsin both act on the polypeptides present in the solution, hence give clear liquid by digesting the albumin contents. Pepsin acts good at pH 2 and Trypsin (or chymotrypsin) acts good pH 8.

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