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Cytochrome C Release Increase in Caspase 3 PARP Cleavage Chromatin Condensation

ID: 161793 • Letter: C

Question

Cytochrome C Release Increase in Caspase 3 PARP Cleavage Chromatin Condensation DNA Fragmentation

If you were to do a timed experiment to determine what happened first, second, third…… How would you do it?

Thursday

8:00am ________________________________ ________________________________ 10:00am ________________________________ ________________________________ 12:00pm ________________________________ ________________________________ 2:00pm ________________________________ ________________________________ 4:00pm ________________________________ ________________________________

Friday

8:00am ________________________________ ________________________________

Explanation / Answer

Nuclear , cytoplasmic and membrane changes for apoptosis

Apototic parameters                                                          Time

1. increase in caspase 3 activity                                          30 min

2. PARP cleavage                                                                1hr

3. Chromatin condensation                                                   3-5hr

4. DNA fragmentation                                                             5hr

5. Decrease cellular metabolism                                             8-12hr

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