pGLO bacterial transformation lab 1. Regarding genetic transfer, what is the mea

ID: 161844 • Letter: P

Question

pGLO bacterial transformation lab

1. Regarding genetic transfer, what is the meaning of transformation? (1%)

2. What is the “pGLO” used in this lab? (1%)

3. Regarding the working mechanism of this pGLO bacterial transformation lab

a. Draw a flow chart to indicate the key basic steps of this lab, long paragraph will not be accepted (3%)

b. How are cells made “competent” in this lab? (Hint: What chemicals are needed to enable the transformation of cells?) (2%)

c. What else is needed to introduce pGLO plasmid into the E-coli? (Hint: What are those heat shock and cooling steps for?) (2%)

d. How are the four agar plates different from each other in this lab, and what are they for? (6%)

4. What are the safety precautions in this lab (Hint: we are dealing with E-coli)? (2%)

5. In addition to the basic steps, you need to emphasize some crucial steps in the experiment

a. Is there any special technique to streak/spread the bacteria so that they grow more evenly on the agar plate? (1%)

b. Can the same pipette or loop be used in multiple tubes or plates? Why? (1%)

c. What should we do to sterilize the loop or the plate after use? (1%)

d. In order to view the agar plate with/without glowing cells at the end, where would be the best position to label the plates? (1%)

e. How do we determine which plate contains the ‘glowing’ bacteria (1%)

1. Regarding genetic transfer, what is the meaning of transformation? (1%)

genetic transformation refers to the active uptake of free DNA by bacterial cells and the heritable incorporation of its genetic information.

2. What is the “pGLO” used in this lab? (1%)

The pGLO is the plasmid used in biotechnology laboratory as a vector for creating genetically modified organisms.

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.