Describe the structure the RuVA tetramer, and explain how it binds and maintains

Question

Describe the structure the RuVA tetramer, and explain how it binds and maintains the Holliday structure during recombination. in your description, include the structure of the RuvA monomer, the features that bind dsDNA, the features that bind ssDNA, and the features that separate base pairs during branch migration. using correct terminology locate these features in the Ruva tetramer. write your description as if the person reading it has never seen the structure, and be sure to arrange the words in your own way.

Explanation / Answer

RuvA :

it is a DNA-binding protein that ties Holliday intersections with high proclivity.

The structure of the complex has been differently illustrated through X-beam crystallography and EM information, and propose that the perplexing comprises of it is possible that maybe a couple RuvA tetramers, with charge lined furrows through which the approaching DNA is diverted.

The structure likewise demonstrated the presence of 'acidic pins' in the focal point of the tetramer, which serve to isolate the DNA duplexes. Its precious stone structure has been tackled at 1.9A.

The RuvA monomer has a "L" shape, made out of three spaces: I, II, III. The circle interfacing space II and area III is not appeared.

domain I: it is a six-stranded, hostile to parallel beta barrel, while both areas II and III comprise completely of alpha helices.

RuvA Tetramer-Holliday Junction DNA

In tetrameric RuvA, every monomer is a flap of the symmetrical tetramer. Area I of every monomer is situated midway, and areas II and III are found incidentally.

Looking at the charge circulation of molecules over the surface of the RuvA tetramer, obviously the DNA restricting surface is to a great extent decidedly charged (fundamental), while the inverse surface is to a great extent adversely charged (acidic). The yellow, orange, and red hues speak to moderately adversely charged iotas and the green and blue hues speak to all the more decidedly charged particles (acidic buildups and fundamental deposits are shaded completely). The relative positive charge of the DNA restricting surface pulls in the adversely charged DNA spine.

A special case to the for the most part fundamental surface of the DNA restricting side of the RuvA tetramer is an eight deposit acidic focal stick including glutamate55 and aspartate56 of every monomer. This contrarily charged focus may repulse the adversely charged oxygens of the DNA spine, pushing the DNA far from the focal point of the Holliday intersection.

Various deposits that communicate straightforwardly or in a roundabout way (by means of water particles) with the DNA spine line the diverts in which Holliday intersection DNA is bound.

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