Alterations in the gut microbiome are known to be associated with certain diseas

Question

Alterations in the gut microbiome are known to be associated with certain diseases. How would you determine what bacteria are differentially present in a diseased vs normal state?

a. You would look for toxins in the stool of diseased individuals compared to normal individuals

b. You would conduct proteomics analysis on the gut microflora from normal compared to diseased subjects

c. You would conduct 16S rDNA sequencing on samples from the gut microflora from normal compared to diseased subjects

d. You would attempt to culture the bacteria from samples of the gut microflora collected from normal and diseased subjects

Explanation / Answer

The gut microbiota in normal and diseased humans play a vital role. The gut microbiota help to reduce pathogenic microbial populations by competition. The gut microbiota help to synthesize specific vitamins in the gut. Gut microbiota produce certain substances that eliminate the pathogenic organisms. Gut microbiota are identified by 16S rDNA sequencig in normal and diseased individuals. Comparision of sequences provide the variation in pathogenic and normal gut microbiota. Option c is correct.

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